The work presented in this thesis ultimately provides a

Autoethanogenum without the requirement for additional constraints. The model has been used to investigate pH and CO supply induced metabolic shifts, uncovering experimentally tested bioprocess conditions which promote ethanol and 2,3 butanediol production. The genes encoding Nfn were identified as knock out targets for improved ethanol production, however the application of a new thermodynamic analysis reveals difficulties which were confirmed experimentally.The work presented in this thesis ultimately provides a demonstrably useful tool for metabolic and bioprocess engineering of C.

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steriods The effect of biochar addition on the levels of black carbon (BC) and polcyclic aromatic hydrocarbons (PAHs) in a vineyard soil in central Italy was investigated within a two year period. Hydropyrolysis (HyPy) was used to determine the contents of BC (BCHyPy) in the amended and control soils while the hydrocarbon composition of the semi labile (non BCHyPy) fraction released by HyPy was determined by gas chromatography mass spectrometry, together with the solvent extractable PAHs. The concentrations of these three polycyclic aromatic carbon reservoirs, changed and impacted differently on the soil organic carbon over the period of the trial. steriods

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